文章摘要
张敬艳,刘庆慧,黄倢.vp28重组核酸疫苗对凡纳滨对虾抗WSSV感染的免疫反应和保护效果.渔业科学进展,2012,33(3):77-82
vp28重组核酸疫苗对凡纳滨对虾抗WSSV感染的免疫反应和保护效果
Protection efficacy of vp28 DNA vaccine and immune responses in Litopenaeus vannamei against white spot syndrome virus
投稿时间：2011-07-02  修订日期：2011-11-30
DOI：
中文关键词: p28  疫苗  凡纳滨对虾  基因调控
英文关键词: vp28  Vaccine  Litopenaeus vannamei  Gene regulation
基金项目:对虾行业专项（201103034）和国家虾产业体系（nycytx-46）共同资助
作者单位
张敬艳 上海海洋大学，201306
农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所，青岛266071 
刘庆慧 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所，青岛266071 
黄倢 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所，青岛266071 
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中文摘要:
      利用改构的pEGFP-N1-ie1强启动子载体构建含vp28基因的核酸疫苗，通过肌肉注射和饲料添加的形式免疫对虾，分析各实验组相对保护率和免疫基因（Dicer,Argonaute,STAT和Lyzome）表达，研究构建vp28重组核酸疫苗对凡纳滨对虾的保护效果。RT PCR结果表明，注射和口服vp28组均可在对虾鳃组织中检测到vp28基因表达。在注射组中vp28的相对保护率为22.4%，饲料免疫组中vp28的相对保护率可达到36.84%。相对于对照组，vp28的注射组的STAT基因和Dicer基因表达量于第7天达到最大值。Argonaute基因表达量于第3天达到最大值，以后呈下降趋势。溶菌酶基因表达量一直呈相对较高的趋势。vp28的饲料投喂组的STAT基因和Argonaute基因表达量于第7天达到最大值，以后呈下降趋势。Dicer基因表达量第3天达到最大值。实验结果表明，构建的vp28核酸疫苗在对虾抗WSSV防治中具有潜在应用价值。
英文摘要:
      In this study, pEGFP-N1-ie1 was reconstructed by inserting WSSV immediate early gene, and vp28 was cloned in a new constructed vector. The survival rates and antiviral associated factors (Dicer, Argonaute, STAT and Lyzome) were detected in Litopenaeus vannamei after immunity by injection and oral-feeding. RT-PCR results showed that vp28 was detected in gill of shrimp by injection and oral-feeding immunity treatment. The relative protection rate of vp28 group reached 22.4% (injection group) and 36.84% (oral feeding group).The STAT and dicer mRNA expression in vp28 group were significantly higher than that of control group after injection treatment for 7 d. The argonaute mRNA expression in vp28 group was significantly higher than that of control group after injection treatment for 3 d and then decreased. The lysozyme mRNA expression in vp28 injection group was consistently significantly higher than that of control group. But the STAT and Argonaute mRNA expression in vp28 group was significantly higher than that of control group on the 7th day of oral-feeding treatment and then decreased. The dicer mRNA expression in vp28 group was significantly higher than that of control group on the 3rd day of oral treatment. These results demonstrated that vp28 DNA vaccine has a potential utility for control of WSSV disease in shrimp.
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